Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy

Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (10): 1970.

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Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy

SUN Zhi-Ling1*, GAO Jue-Min2, XU Zhi-Yang3, WANG Zheng4, WANG Fu-Qiang3

1. School of Nursing, Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China;
2. Department of Neurosurgery, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;
3. Center of Instrument, Nanjing Medical University, Nanjing 210029, China;
4. Urban Science Department, Jiangsu Radio and TV University, Nanjing 210017, China

Received:2010-04-09 Online:2010-10-10 Published:2010-10-10
Contact: SUN Zhi-Ling. E-mail:szl@njutcm.edu.cn
Supported by:
江苏省自然科学基金(批准号: BK2009428)、江苏省高校自然科学基础研究项目(批准号: 08KJB320010)及南京中医药大学基础研究与重点培育专项基金(批准号: 10XPY04)资助.

Abstract

Abstract: To explore the mechanism of epilepsy, the proteins that were differentially expressed in the epilepsy and normal tissue were studied. Two-dimensional gel electrophoresis was performed to separate differentially expressed proteins that were subsequently identified by mass spectrometry. Totally 2500—3000 spots were detected in samples, 21 significantly differentially expressed spots were selected, and 17 proteins were identified. Two proteins in epilepsy are up-regulated, 15 proteins are down-regulated. Part of the identified protein candidates have been reported to be associated with epilepsy. However, some of the proteins identified in current were for the first time revealed to be linked to epilepsy. The functions of these proteins, the role of them in epilepsy were discussed.

Key words: Epilepsy, Two-dimensional gel electrophoresis, Mass spectrometry, Proteomics

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SUN Zhi-Ling*, GAO Jue-Min, XU Zhi-Yang, WANG Zheng, WANG Fu-Qiang. Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy[J]. Chem. J. Chinese Universities, 2010, 31(10): 1970.

References

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Proteomic Screening of the Differentially Expressed Proteins Related with Epilepsy

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