Superoxide Biosensor Based on Superoxide Dismutase/Nano-ZnO

Abstract

Abstract: A nanoporous ZnO film(nano-ZnO) was prepared by physical vapor deposited method and its physical and chemical properties were studied by means of SEM, UV-Vis, XRD and electrochemical methods. The results show that nano-ZnO is a nanoporous film with a polycrystalline hexagonal wurtzite structure. The average diameter of the nanoparticles is about 50—100 nm and its band gap is 3.37 eV. A new superoxide dismutase(SOD) modified nano-ZnO electrode(SOD/ZnO) was prepared by dipping method. SOD was modified stably in the nano-ZnO film confirmed by electrochemical impedance spectroscopy(EIS) and the direct electron transfer could be achieved confirmed by cyclic voltammetry(CV). Spectrum studies indicated that SOD kept its bio-activities. A novel third-generation O₂^- biosensor with high selectivity, quick response time(4 s), large linear range(anodic current response: 0.24—180×10^-6 mol/L; cathodic current response: 0.12—250×10^-6 mol/L) and good sensitivity(anodic: 2×10^-7 mol/L; cathodic: 1×10^-7 mol/L) was constructed and its analytical properties were studied.

Key words: ZnO, Superoxide dismutase, Superoxide anion, Biosensor

CLC Number:

O657.1

TrendMD:

DENG Zi-Feng, XU Wei, YANG Xiao^*. Superoxide Biosensor Based on Superoxide Dismutase/Nano-ZnO[J]. Chem. J. Chinese Universities, doi: .

References

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