Studies on the Biotransformation of Aconitine in Human Intestinal Bacteria Using Soft-ionization Mass Spectrometry

Chem. J. Chinese Universities

• 研究论文 • Previous Articles Next Articles

Studies on the Biotransformation of Aconitine in Human Intestinal Bacteria Using Soft-ionization Mass Spectrometry

ZHAO Yu-Feng^1,2, SONG Feng-Rui¹, GUO Xin-Hua¹, LIU Shu-Ying¹*

1. Changchun Center of Mass Spectrum, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China

Received:2007-05-09 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
Contact: LIU Shu-Ying

Abstract

Abstract: To study the biotransformation of aconitine in human intestine, aconitine was incubated with human intestinal bacteria in vitro. Aconitine and its metabolites formed the protonated molecule [M+H]⁺ in positive ion mode. According to the m/z of [M+H]⁺, the metabolites of aconitine were investigated by electrospray ionization tandem mass spectrometry directly. Aconitine can be transformed by human intestinal bacteria. Aconitine was converted into more than twenty kinds of new metabolites such as mono-ester aconitum alkaloids, diester aconitines and lipo-alkaloids by deacetylation, dehydroxylation, demethylation and esterification.

Key words: Aconitine, Human intestinal bacteria, Biotransformation, Electrospray ionization mass spectrometry

CLC Number:

O657

TrendMD:

ZHAO Yu-Feng^1,2, SONG Feng-Rui¹, GUO Xin-Hua¹, LIU Shu-Ying¹*. Studies on the Biotransformation of Aconitine in Human Intestinal Bacteria Using Soft-ionization Mass Spectrometry[J]. Chem. J. Chinese Universities, doi: .

References

Related Articles 15

[1]	LUO Lei, MU Xiaoqing, WU Tao, NIE Yao, XU Yan. Synthesis of Norephedrine in One Pot [J]. Chem. J. Chinese Universities, 2021, 42(8): 2458.
[2]	DENG Jiewei, YANG Yunyun, LIN Li, LUAN Tiangang. Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics [J]. Chem. J. Chinese Universities, 2020, 41(9): 2011.
[3]	LI Xiaoqian, ZHANG Hua, LU Haijian, LIU Chang, LIU Qinglong, MA Xiayu, FANG Yuanping, LIANG Dapeng. Mechanism of Photocatalytic Degradation of Rhodamine B by TiO₂ Nanowire Array with Internal Extraction Electrospray Ionization Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 2003.
[4]	ZHU Ling,WANG Yuchen,ZHAO Jiangyuan,WEN Mengliang,LI Minggang,HAN Xiulin. Transformation of Ginsenoside Rb₃ and C-Mx by Recombinant β-Xylosidase ^† [J]. Chem. J. Chinese Universities, 2020, 41(5): 1010.
[5]	WANG Xiaoqun, HUANG Guangming. Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging [J]. Chem. J. Chinese Universities, 2020, 41(12): 2673.
[6]	DU Chenhui, LI Ze, CUI Xiaofang, ZHANG Min, PEI Xiangping, ZHAN Haixian, YAN Yan. Chemical Transformation of Raw and Fermented Ziziphi Spinosae Semen Analyzed by UPLC-Q-Orbitrap MS/MS [J]. Chem. J. Chinese Universities, 2019, 40(8): 1614.
[7]	HAN Mingxin, LI Fangtong, ZHANG Yan, DAI Yulin, ZHENG Fei, YUE Hao. Biotransformation of Rare Protopanaxadiol Saponin by Human Intestinal Microflora^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1390.
[8]	XIAO Yongkun,LIU Chunying,YU Hongshan,YI Tea-Hoo,XU Longquan,SONG Jianguo,IM Wan-Teak,SUN Changkai,JIN Fengxie. Dynamic Biotransformation of Protopanaxadiol-ginsenosides and Preparation of Minor Ginsenosides C-K or $F 2 †$ [J]. Chem. J. Chinese Universities, 2019, 40(6): 1184.
[9]	LI Cheng,WANG Chengjian,JIN Wanjun,HAN Jianli,YANG Meifang,GAO Xi,HUANG Linjuan,WANG Zhongfu. Mass Spectrometric Analysis of N-Glycans of Glycoprotein Separated by SDS-PAGE Gel from Ginkgo Seed^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 69.
[10]	WU Fangling,CHU Yanqiu,CHEN Xin,WEI Wanghui,DING Chuanfan. Critical Factors Affecting Noncovalent Interaction Between Pentapeptides Explored by Electrospray Ionization Mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2018, 39(9): 1927.
[11]	QIAO Mengdan, LIU Shang, ZHANG Yan, LI Jing, ZHENG Fei, DAI Yulin, YUE Hao. Biotransformation of Ginsenosides in Fermented Ginseng Using UPLC-Q-Orbitrap MS/MS^† [J]. Chem. J. Chinese Universities, 2018, 39(2): 219.
[12]	LIU Xinru, LIU Chunying, XU Longquan, SONG Jianguo, YU Hongshan. Construction of Ginsenoside-β-glucosidase Gene Vector and Biotransformation in Pichia Pastoris^† [J]. Chem. J. Chinese Universities, 2018, 39(11): 2451.
[13]	LI Ruigang,ZHU Na,ZHAO Huanxi,WANG Nan,SUN Hongmei,YUE Hao,LI Jing. Effects of Ginseng Polysaccharides on the Metabolism of Ginsenoside Re in vivo and Transformation of Ginsenoside Re in vitro^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2192.
[14]	KE Mufang, HAN Jing, ZHU Tenggao, LIU Wenjie, ZHANG Hua, KOU Wei, LIANG Dapeng. Direct and Rapid Analysis of Avocado Using Internal Extractive Electrospray Ionization Mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2017, 38(5): 738.
[15]	HUANG Yandong, WU Ruofei, CHU Yanqiu, DING Chuanfan. Effect of Side Chain of α-Amino Acids and Esters on the Stability Constants for β-Cyclodextrin Complexes^† [J]. Chem. J. Chinese Universities, 2017, 38(5): 743.

Studies on the Biotransformation of Aconitine in Human Intestinal Bacteria Using Soft-ionization Mass Spectrometry

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments