In vitro and in vivo Activation of Mouse Liver Astral Cell by Confocal Raman Microspectroscopy

Chem. J. Chinese Universities

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In vitro and in vivo Activation of Mouse Liver Astral Cell by Confocal Raman Microspectroscopy

GOAN Il-Ho^1,2, SHEN Ai-Guo^1,2, LIAO Zhang-Xiu³, WANG Hui³, HU Ji-Ming^1,2*

1. Institute of Analytical Biomedicine,
2. College of Chemistry and Molecular Sciences,
3. College of Basic Medicine, Wuhan University, Wuhan 430072, China

Received:2006-12-27 Revised:1900-01-01 Online:2007-09-10 Published:2007-09-10
Contact: HU Ji-Ming

Abstract

Abstract: The feasibility of a novel and efficient diagnostic method for liver fibrosis via Raman spectroscopy was studied. Confocal Raman Spectroscopy(CRS) was utilized to monitor the molecular changes of rat hepatic stellate cells(HSCs) in vitro as well as in vivo activation. In vitro activation was induced by growth in uncoated plastic plates while the in vivo activation was induced by a single intraperitoneal injection of carbon tetrachloride(CCl₄). Raman spectroscopy in combination with biological method perfectly established a correlation between spectral changes and morphologic changes within HSCs. The biochemical changes of HSCs during the activation such as the loss of retinoid, the increase of a-helical protein, and the increased production of extracellular matrix proteins were observed by CRS. Raman spectroscopy combined with fiber optical probe could be potentially accomplished in vivo detection, which can lead to a novel and efficient diagnosis for liver fibrosis.

Key words: Hepatic stellate cells, In vivo/in vitro activation, Biochemical change, Raman spectroscopy

CLC Number:

O657.37

TrendMD:

GOAN Il-Ho^1,2, SHEN Ai-Guo^1,2, LIAO Zhang-Xiu³, WANG Hui³, HU Ji-Ming^1,2*

. In vitro and in vivo Activation of Mouse Liver Astral Cell by Confocal Raman Microspectroscopy[J]. Chem. J. Chinese Universities, doi: .

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In vitro and in vivo Activation of Mouse Liver Astral Cell by Confocal Raman Microspectroscopy

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