Application of HPLC-ESI-MS/MS to the Determination of a Novel Antitumour Platinum Complex

Chem. J. Chinese Universities

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Application of HPLC-ESI-MS/MS to the Determination of a Novel Antitumour Platinum Complex

SU Qiang1,2, LIU Qing-Fei², LIANG Qiong-Lin³, WANG Yi-Ming^2,3, BI Kai-Shun¹, LIU Wei-Ping⁴, LUO Guo-An^2,3*

1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;2. Institute of Life Science and Biomedicine, 3. Department of Chemistry, Tsinghua University, Beijing 100084, China; 4. Kunming Institute of Precious Metals, Kunming 650221, China

Received:2006-11-23 Revised:1900-01-01 Online:2007-08-10 Published:2007-08-10
Contact: LUO Guo-An

Abstract

Abstract: A novel platinum complex, named as saliplatin, was designed, synthesized and characterized with element analysis, coupled inductively plasma-mass spectrum, gravitational thermal-differential thermal analysis, ultraviolet-visible absorption spectrum, infrared spectrum, fast atom bombment mass spectrum and ¹H NMR, etc. Its purity was investigated and determined. HPLC-ESI-MS was employed to separate the platinum complex and the impurity, and to identify and quantify the latter. It proved that the impurity in this novel compound was 3,5-diisopropylsalycilic acid, a reactant used in the synthesis of the novel platinum complex, identified with ESI-MS. The mass fraction was determined with HPLC as 4.3%, which meant that the purity of this new substance was 95.7%.

Key words: ESI-MS, Platinum complex, Antitumour drug

CLC Number:

O657.6

TrendMD:

SU Qiang1,2, LIU Qing-Fei², LIANG Qiong-Lin³, WANG Yi-Ming^2,3, BI Kai-Shun¹, LIU Wei-Ping⁴, LUO Guo-An^2,3*. Application of HPLC-ESI-MS/MS to the Determination of a Novel Antitumour Platinum Complex[J]. Chem. J. Chinese Universities, doi: .

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Application of HPLC-ESI-MS/MS to the Determination of a Novel Antitumour Platinum Complex

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