Abstract:

Traditional TiO₂ is bioinert, which could just form mechanical connection with the surrounding bone-tissue without bioactivity. Because of the nano-effect of nano-materials, the properties of nano-material is always better than the traditional ones, which made it logical to assume that nano-technology is a potential method to improve the bioactivity of ceramics. In this paper, the bioactivities of nano-TiO₂ ceramics prepared via normal sintering method by adding hydroxyapatite(HA) or MgO as the crystal growth inhibitors were studied with cell culture method, in which Ros17/28(osteoblast) was cultured on nano-ceramics in vitro. The bioactivity and biocompatibility of nano-TiO₂ ceramics were evaluated by investigating the cell extrinsic shape, integrality of cell, cell activity and proliferation by SEM observation and cell toxicity tests including fluorescence coloration observation and MTT, respectively. The result shows that nano-TiO₂ ceramics with HA as the crystal growth inhibitor had a good biocompatibility. Its bioactivity is better than pure HA and TiO₂ ceramics with MgO as the crystal growth inhibitor.

Key words: Nano-TiO₂ ceramics, Osteoblast, Cell culture, Bioactivity