Divalent Metal Ion-activated DNA Cleavage Activity of Copper Superoxide Dismutase

Chem. J. Chinese Universities

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Divalent Metal Ion-activated DNA Cleavage Activity of Copper Superoxide Dismutase

ZHOU Ruo-Yu¹, HAN Ying-Chun¹, JIANG Wei¹, YANG Hao¹, LIU Chang-Lin²

1. Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074;
2. Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, Central China Normal University, Wuhan 430079, China

Received:2006-06-02 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10
Contact: LIU Chang-Lin

Abstract

Abstract: The study examined the DNA cleavage activity mediated by each member of the Cu²⁺-containing forms of CuZnSOD(Cu_nSOD, n=1—4) in the presence of exogenous Mg²⁺ or Mn²⁺. The result indicates that upon addition of Mg²⁺ or Mn²⁺, Cu_nSOD can more efficiently cleave DNA via a hydrolytic pathway compared with its holo- and apo-forms. The factors, such as exogenous divalent metal concentration, pH and enzyme concentration, can affect the DNA cleavage activity. We also measured the relative enzyme activity and the steady state kinetic parameters of the DNA cleavage reaction, indicating that the DNA cleavage activity is independent of Cu²⁺ number in Cu_nSOD, and increased in an order of Cu₁SOD2SOD≈Cu₄SOD3SOD.

Key words: CuZnSOD, Cu_nSOD, Hydrolysis, DNA cleavage

CLC Number:

O614

TrendMD:

ZHOU Ruo-Yu¹, HAN Ying-Chun¹, JIANG Wei¹, YANG Hao¹, LIU Chang-Lin². Divalent Metal Ion-activated DNA Cleavage Activity of Copper Superoxide Dismutase[J]. Chem. J. Chinese Universities, doi: .

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Divalent Metal Ion-activated DNA Cleavage Activity of Copper Superoxide Dismutase

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