Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (4): 632.

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Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes

SUN Yue¹, LU Min², YIN Xue-Feng^1*

1. Institute of Microanalytical Systems, Department of Chemistry; 2. Institute of Biologic Macromolecule and Enzymatic Engineering, College of Life Science, Zhejiang University, Hangzhou 310027, China

Received:2005-12-20 Online:2006-04-10 Published:2006-04-10
Contact: YIN Xue-Feng,E-mail:yinxf@zju.edu.cn

Abstract

Abstract: A new method that nanometer-liposomes could penetrate the cell membrane to deliver their encapsulated material into cells was investigated. Nanometer sized-liposomes were produced from phosphatidylcholine to encapsulate fluorescent dyes and FITC labeled superoxide dismutase(SOD-FITC) by an ultrasonic method. The size distribution of liposomes was determined with a Laser Particle Analyser. The fluorescence images demonstrate that liposomes can transfer fluorescent dyes(FITC, Rhodamine B), which are not cell membrane permeable, into cells. Single cell analysis of the delivered SOD-FITC with microchip electrophoresis revealed that the density of liposomes and interaction-time between liposomes and cells affected the efficiency of intracellular delivery of encapsulated material.

Key words: Single cell analysis, Liposome, Intracellular derivatization, Fluorescent dye

CLC Number:

O657

TrendMD:

SUN Yue, LU Min, YIN Xua-Feng. Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes[J]. Chem. J. Chinese Universities, 2006, 27(4): 632.

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Intracellular Delivery of Fluorescent Dyes Mediated by Nanometer-Liposomes

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