Establishment and Application of On-line Parallel-trap SFE-HPLC System

Chem. J. Chinese Universities

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Establishment and Application of On-line Parallel-trap SFE-HPLC System

ZHANG Jie¹, LIANG Zhen¹, ZHANG Li-Hua¹, ZHANG Wei-Bing¹, HUO Yu-Shu², ZHANG Yu-Kui¹

1. National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2. Health Science Center at San Antonio, The University of Texas, San Antonio 78229-3900, USA

Received:2006-01-24 Revised:1900-01-01 Online:2006-12-10 Published:2006-12-10
Contact: ZHANG Yu-Kui

Abstract

Abstract: A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC) system was established. The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide, and HPLC was used to analyze the transferred sample. The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum. The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined, which accorded with the result obtained with off-line extraction. Using this system, the whole process can be monitored and the optimum extraction time can be determined with single run. In addition, it has many other advantages, such as low sample loading, high sensitivity and accuracy, short analysis time, avoiding sample pollution and easily operation etc. The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.

Key words: Supercritical fluid extraction, High performance liquid chromatography, Ganoderma lucidum

CLC Number:

O657

TrendMD:

ZHANG Jie¹, LIANG Zhen¹, ZHANG Li-Hua¹, ZHANG Wei-Bing¹, HUO Yu-Shu², ZHANG Yu-Kui¹. Establishment and Application of On-line Parallel-trap SFE-HPLC System[J]. Chem. J. Chinese Universities, doi: .

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Establishment and Application of On-line Parallel-trap SFE-HPLC System

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