Abstract: The adenine, 8-hypoxanthine, 6-thioguanine and 8-azaguanine were separated and determined with the method of micellar electrokinetic capillary chromatography. The optimal conditions of electrophoresis were studied. At 25 ℃ and 20 kV applied voltage, the four components were baseline separated within 16 min in 25 mmol/L phosphate-borate buffer(pH=7.0) including 30 mmol/L SDS at the detection wavelength of 210 nm. The peek area versus concentration was linear over the concentration range of (12-200)×10^-6 mol/L for 8-hypoxanthine and 8-azaguanine, and (6.5-100)×10^-6 mol/L for adenine and 6-thioguanine. The detection limits of all analytes mentioned above attained to 10^-6 mol/L grade. And the correlationg coefficients of four analytes were over 0.998 5. In the real sample of the mean absolute recoveries of 8-hypoxanthine, adenine, 6-thioguanine and 8-azaguanine were 97.2%-102.1%, and the relative standard deviations for the four components were less than 3.41%(n=5). The method is rapid, simple with a good solution for the analysis of 8-hypoxanthine, adenine, 6-thioguanine and 8-azaguanine, and satisfactory results were obtained.

Key words: Micellar electrokinetic capillary chromatography, Adenine, 8-Hypoxanthine, 6-Thioguanine, 8-Azaguanine