DNA Electrochemical Biosensor Based on Functional Gold Nanoparticles-amplification

Abstract

Abstract: Ferrocene(Fc)-capped gold nanoparticle/streptavidin conjugates were attached onto the electrodes covered with either biotinylated or sandwich oligodeoxynucleotides(ODNs) duplexes. The binding events were monitored by electrochemical signal of ferrocene. The signal differences permitted to distinguish the match of two perfectly complementary DNA strands from the near-perfect match where just one base pair was mutant. The effects of the DNA probe and target strand lengths on the voltammetric behavior of the Fc groups were studied. It was found that shorter duplexes would lead to a more reversible voltammetric wave and a higher detection level. For the analysis of 30-mer biotinylated target DNA, a concentration level as low as 2.5×10^-4 nmol/L could be detected while for 39-mer target of sandwich duplexes, the limit of detection was 2.0×10^-3 nmol/L. The capabilities of the method for trace analysis of DNA can be attributed to the enhanced voltammetric detection by the large number of Fc moieties present in each conjugate.

Key words: DNA, Functional gold nanoparticles, Ferrocene, Cyclic voltammetry

CLC Number:

O657

TrendMD:

LI Jin-Hua, HU Jing-Bo, DING Xiao-Qin, LI Qi-Long . DNA Electrochemical Biosensor Based on Functional Gold Nanoparticles-amplification[J]. Chem. J. Chinese Universities, 2005, 26(8): 1432.

References

[1] Palecek E.,Fo jta M..Anal.Chem.[J],1994,66:1565—1569 [2] Millan K.M.,Alek sand rs J.S..Electroanalysis[J],1992,4:929—932 [3] Nakano K.,Maeda M..Anal.Sci.[J],1997,13:455—456 [4] Millan K.M.,Angela S.,Su san R.M..Anal.Chem.[J],1994,66:2943—2947 [5] Jacob s K.,Wo lf S.F.,Haines L.et al..Nucle.iAcid s Res.[J],1987,15:2911—2915 [6] Chee M.,Yang R.,Hubbell E.et al..Science[J],1996,274:610—615 [7] XU Chun(徐春),CA I Hong(蔡宏),H E Pin-Gang(何品刚)et al..Chem.J.Ch inese Un iversities(高等学校化学学报)[J],2001,22(9):1492—1495 [8] CA I Hong(蔡宏),W AN G Yan-Qin(王延琴)H E Pin-Gang(何品刚)et al..Chem.J.Ch inese Un iversities(高等学校化学学报)[J],2003,24(8):1390—1394 [9] Au th ier L.,Gro ssio rd C.,Bro ssier P.et al..Anal.Chem.[J],2001,73:4450—4456 [10] Wang J.,Fo lsky R.,Merkoci A.et al..Langm u ir[J],2003,19:989—991 [11] Pato lsky F.,Weizm ann Y.,Willner I..J.Am.Chem.Soc.[J],2002,124:770—772 [12] Alfon ta L.,Singh A.K.,Willnw e I..Anal.Chem.[J],2001,73:91—102 [13] Kelley S.O.,Boon E.M.Barton I.K.et al..Nucleic.Acid s Res.[J],1999,27:4830—4837 [14] Sauerb rey G.Z..Phys.[J],1959,155:206—210 [15] Creager S.E.,Row e G.K..J.Electroanal.Chem.[J],1994,370:203—211 [16] Wang J.,Li J.H.,Baca A.J.et al..Anal.Chem.[J],2003,75:3941—3945 [17] Hoogvliet J.C.,Dijk sm a M.,Kam p B.et al..Anal.Chem.[J],2000,72:2016—2021 [18] Baca A.J.,Zhou F.M.,Wang J.et al..Electroanalysis[J],2004,16(122):73—80 [19] Caru so F.,Rodda E.,Fu rlong D.N.et al..Anal.Chem.[J],1997,69:2043—2049 [20] Satjap ip ar M.,Saned rin R.,Zhou F.M..Langm u ir[J],2001,17:7637—7644 [21] Ye S.,Sato Y.,Uo sak i K..Langm u ir[J],1997,13:3157—3161 [22] Creager S.E.,Row e G.K..J.Electroanal.Chem.[J],1997,420:291—299 [23] Herne T.M.,Tarlov M..J.Am.Chem.Soc.[J],1997,119:8916—8920 [24] Steel A.B.,Herne T.M.,Tarlov M.J..Anal.Chem.[J],1998,70:4670—4677 [25] Ihara T.,Maruo Y.,Takenaka S.et al..Nucleic.Acid s Res.[J],1996,24:4273—4280 [26] Wang J.,Rivas G.,Fernandes J.R.et al..Anal.Ch im.Acta[J],1998,375:197—203 [27] Arm istead P.M.,Tho rp H.H..Anal.Chem.[J],2001,73:558—564 [28] Kan Y.W.,Chehab F.F..Proc.Natl.Acad.Sci.U.S.A.[J],1989,86:9178—9182 [29] Zhang Y.C.,Kin H.H.,Mano N.et al..Anal.Bioana.lChem.[J],2002,374:1050—1055