Preparation of Ion Exchange Mesoporous Sieve DEAE-SBA-15 and Its Application to Separation and Purification of Der f 2 Allergen in Dermatophagoides Farinae

Abstract

Abstract: Mesoporous silica SBA-15 was functionalized by 2-diethylamino-ethanol(DEAE) through the (epoxide) ring opening of 3-glycidyloxy-propyl-trimethoxy silane grafted on the silica surface(denoted as DEAE-SBA-15). Subsequently, DEAE-SBA-15 was used as the weak anion exchange matrix for chromatography to purify (Der f 2) allergen from the spent culture medium extract of Dermatophagoides farinae, one of House dust mites that can cause heavy atopic diseases such as asthma and dermatitis. The structure of DEAE-SBA-15 was confirmed by ¹³C and ²⁹Si CP MAS NMR and other methods. By SDS-PAGE, Der f 2 was estimated to have a molecular weight of 14 000 and appeared as the only separated protein band. The assay results of skin prick test showed that the allergic activity of Der f 2 was retained. The above results indicate that DEAE-SBA-15 materials are promising fixed-matrix candidates in liquid chromatography to purify proteins.

Key words: DEAE-SBA-15, Stationary phase preparation, Dermatophagoides farinae, Purification, Der f 2 allergen

CLC Number:

O614

TrendMD:

LEI Jie, ZHANG Lu-Yan, DING Sheng-Song, JIANG Shi-Yi, YU Cheng-Zhong, ZHAO Dong-Yuan . Preparation of Ion Exchange Mesoporous Sieve DEAE-SBA-15 and Its Application to Separation and Purification of Der f 2 Allergen in Dermatophagoides Farinae[J]. Chem. J. Chinese Universities, 2005, 26(5): 793.

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Preparation of Ion Exchange Mesoporous Sieve DEAE-SBA-15 and Its Application to Separation and Purification of Der f 2 Allergen in Dermatophagoides Farinae

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