Abstract: Mb/AAO/Au colloid /GC electrodes were made by immobilizing Mb on the surface of Au colloid modified glassy carbon electrode covered with AAO templates.The cyclic voltammgram of Mb/AAO/Au colloid/GC films shows a pair of well-defined and nearly reversible peaks at about-0.21 V(vs.Ag/AgCl) in acetic acid-sodium acetate buffers with pH=5.4,being the characteristic peaks of the Mb heme Fe(Ⅲ)/Fe(Ⅱ) redox couples.In the microenvironment of the AAO/Au colloid film,the electron transfer between Mb and the electrode was greatly enhanced.The hydrogen peroxide and oxygen were catalytically reduced at(Mb/AAO/Au) colloid/GC electrodes.

Key words: Myoglobin(Mb), Nano-alumina, Gold colloid, Direct electrochemistry, Electrocatalysis