Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain

Abstract

Abstract: A mutated strain of Streptococcus zooepidemicus capable of producing the polysaccharide in a high yield was obtained after being mutated bythe chemical agent N-methyl-N′-nitro-N- nitrosoguanidine(NTG). Polysaccharide produced bythe mutated strain of Streptococcus zooepidemicus was purified bythe procedure including Savage method, quaternary ammonium compound precipitation, DEAE-cellulose(DE52) column chromatograph and Sephadex G-75 gel filtration. The purified polysaccharide was proved to be hyaluronic acid(HA) by means of chemical composition analysis, ¹³C NMR, Infrared spectra, and circular dichroism. The single helix conformation of the purified HA was studied by Congo red experiment. The molecular weight of the HA was about 1.16×10⁶, which was measured by viscosity method.

Key words: Streptococcus zooepidemicus, Hyaluronic acid, Separation and purification, Characterization

CLC Number:

O657

TrendMD:

HONG Shui-Sheng, CHEN Jia, TENG Li-Rong, MENG Qing-Fan, ZHANG Jian-Guo, LIU Lan-Ying . Purification and Structure Characterization of the Hyaluronic Acid Produced by a Streptococcus Zooepidemicus Mutation Strain[J]. Chem. J. Chinese Universities, 2004, 25(5): 853.

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