Modulation of Urinary Macromolecules Induced by DPPC Monolayers on Morphology and Crystal Face of Calcium Oxalate Crystals

Abstract

Abstract: The effect of urinary macromolecules such as chondroitin sulfate A(C₄S), chondroitin sulfate C(C₆S) and serum albumin(SA) on urinary crystal calcium oxalate grown beneath dipalmitoylphosphatidylcholine (DPPC) monolayer was investigated by using scanning electron microscopy (SEM), X-ray powder diffraction (XRD) and Fourier transform infrared analysis(FTIR). Only the calcium oxalate monohydrate (COM) was nucleated and grown beneath the monolayer with the (101) face oriented preferentially towards the monolayer interface. On the monolayer in the absence of additives, the tri-dimensional prismy COM crystals precipitated. However, the (101) face of COM was doubly strengthened and the other faces were weakened in the presence of urinary macromolecule inhibitors. This leads to the formation of two-dimensional plate-like COM crystals. The result is attributed to the interaction between the positively charged calcium-rich (101) face of COM with the negative-charged headgroups of the monolayer and the negatively charged macromolecules. All the three additives can inhibit the growth of COM crystals.

Key words: Calcium oxalate, Urinary macromolecules, Crystal face, Monolayer, Urinary calculi

CLC Number:

O614.23

TrendMD:

OUYANG Jian-Ming, DENG Sui-Ping, ZHONG Jiu-Ping . Modulation of Urinary Macromolecules Induced by DPPC Monolayers on Morphology and Crystal Face of Calcium Oxalate Crystals[J]. Chem. J. Chinese Universities, 2004, 25(5): 802.

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