Determination of Multipeptide Mixture by Using CZE Coupled with ESI-MS

Abstract

Abstract: CZE ESI MS is one of the most sensitive detection method and one of the potential method for protomics analysis. In this work, peptide mixture was separated and detected by using CZE ESI MS, and the influence of pH value of buffer and composition of sheath liquid on CE separation and MS detection was investigated. The method allows the detection limits up to 4.2—33 pg. The result of this method is better than that of CE UV for the peptide analysis.

Key words: Capillary electrophoresis, Peptide, Mass spectrometry, Sheath liquid, Electrospray ionization interface

CLC Number:

O657

TrendMD:

LIANG Zhen, ZHANG Wei-Bing, ZHANG Yu-Kui . Determination of Multipeptide Mixture by Using CZE Coupled with ESI-MS[J]. Chem. J. Chinese Universities, 2004, 25(3): 448.

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Determination of Multipeptide Mixture by Using CZE Coupled with ESI-MS

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