Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H2O2 Using Stopped-flow Time-resolved Spectrophotometry

Abstract

Abstract: Interaction between oxyhemoglobin and H₂O₂ was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H₂O₂ suggested the presence of an intermediate species. The influence of pH, concentration of H₂O₂, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.

Key words: Stopped-flow time-resolved spectrophotometry, Oxyhemoglobin, Hydrogen peroxide, Mechanism

CLC Number:

O657

TrendMD:

WANG Rui-Yong, CAI Ru-Xiu, LIN Zhi-Xin . Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H₂O₂ Using Stopped-flow Time-resolved Spectrophotometry[J]. Chem. J. Chinese Universities, 2004, 25(2): 267.

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Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H₂O₂ Using Stopped-flow Time-resolved Spectrophotometry

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