Kinetics Study of Iron Release of Human Serum Ferritin with MALDI-TOF Mass Spectrometry and Electronic Spectrum Technology

Abstract

Abstract: MALDI-TOF mass spectrometry was used to understand the compound of proteins in human serum and electronic spectrum was used to study the kinetics of iron release of the human serum ferritin. The result shows that if Na₂S₂O₄ was taken as the reducer, it is impossible to examine the iron release in serum with normal spectrum. When Vc was used as the reducer, the kinetic reaction of the iron release from serum ferritin was the first-order. It might be due to the abundance and influence of other proteins in human serum in the course of iron release, and that could fit the need of iron in the body.

Key words: Serum, Ferritin, Iron release, Kinetics, Mass spectrometry

CLC Number:

O617

TrendMD:

YAN Li, HUANG He-Qing, JIN Hong-Wei, CHEN Ping, YANG Tian-Ci, WANG Qun-Li . Kinetics Study of Iron Release of Human Serum Ferritin with MALDI-TOF Mass Spectrometry and Electronic Spectrum Technology[J]. Chem. J. Chinese Universities, 2004, 25(10): 1889.

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Kinetics Study of Iron Release of Human Serum Ferritin with MALDI-TOF Mass Spectrometry and Electronic Spectrum Technology

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