Direct Electrochemistry of Hemoglobin Immobilized on Carbon Paste Electrode by Inorganic Film

Abstract

Abstract: Direct electrochemical behavior of hemoglobin (Hb) immobilized on carbon paste electrodes by Sol-gel film derived from tetraethylorthosilicate was investigated for the first time. Hb-Sol-gel-modified electrodes showed a pair of well-defined and nearly reversible cyclic voltammetric peaks for HbFe(Ⅲ)/ Fe( Ⅱ ) redox couple at about -0. 275 V(vs. Ag/AgCl) in pH 7. 0 phosphate buffers. The formal potential of Hb heme Fe( Ⅲ )/Fe(Ⅱ) couple shifted linearly between pH5. 0-11. 0 with a slope of 51. 85 mV/ pH, suggesting that one proton was coupled to each electron transfer in the electrochemical reaction. It was demonstrated by FTIR spectra that the structure of Hb was still maintained native-like in the film. The immobilized Hb displayed the features of a peroxidase and gave an excellent electrocatalytic response to the reduction of H₂O₂ Consequently, this work provides a possible route of using Hb-Sol-gel-modified electrodes as the third generation biosensors.

Key words: Hemoglobin, Silica Sol-gel film, Carbon paste electrode, Direct electrochemistry

CLC Number:

O657
O646

TrendMD:

WANG Quan-Lin, YANG Bao-Jun, LU Gong-Xuan. Direct Electrochemistry of Hemoglobin Immobilized on Carbon Paste Electrode by Inorganic Film[J]. Chem. J. Chinese Universities, 2003, 24(9): 1561.

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Direct Electrochemistry of Hemoglobin Immobilized on Carbon Paste Electrode by Inorganic Film

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