Electrochemical Studies of Reaction Between Dactinomycin and DNA

Abstract

Abstract: The electrochemical behavior of Dactinomycin(Act-D) and the interaction between Act-Dand DNAwere investigated by using electrochemical method.In 0.1 mol/L NaH₂PO₄-Na₂HPO₄buffer(pH=7.0), Act-Dshowed a pair of oxidative and reductive peaks at -0.19 and -0.51 Vin the cyclic voltammetry.Act-Dreacted with DNAand formed an non-electroactive complex, resulting in a decrease of reductive current.The diffusion coefficient of Act-D(D), binding constant(K) and binding site size(s) of Act-Dand DNAwere obtained simultaneously by nonlinear fitting analysis of electrochemical data based on an electrochemical equation for examining the interaction between irreversible redox compounds and DNA.The results were: Df=2.0×10^-5cm²·s^-1, K=5.87×10⁹cm³·mol^-1, s=8.3. The overall results demonstrate that Act-Dbinds tightly to eight base pairs of ct DNA.The two cyclic penta-depsipeptides of Act-Dreinforce the interaction with DNA.

Key words: Dactinomycin, DNA, Electrochemistry method

CLC Number:

O657

TrendMD:

WANG Su-Fen, PENG Tu-Zhi, LI Jian-Ping . Electrochemical Studies of Reaction Between Dactinomycin and DNA[J]. Chem. J. Chinese Universities, 2003, 24(8): 1468.

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Electrochemical Studies of Reaction Between Dactinomycin and DNA

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