Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (5): 900.

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Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining

XU Shu-Ping¹, WANG Lian-Ying², XU Wei-Qing¹, ZHAO Bing¹, YUAN Hang³, MA Lan³, BAI Yu-Bai², FAN Yu-Guo¹

1. Key laboratory for Supramolecular Structure and Material of Minisry of Education of China ;
2. College of Chemistry, Jilin University, Changchun 130023, China;
3. Monoclonal Antibody Technology Center, Yunnan University, Kunming 650091, China

Received:2003-01-13 Online:2003-05-24 Published:2003-05-24

Abstract

Abstract: Aspectral measurement combined with nanoparticle labeling and SERStechnique is described. The gold nanoparticles not only modified by the mouse monoclonal antibody agaist Hepatitis Bsurface antigen [IgG(H)] but also labeled by the p-mercaptobenzoic acid (MBA) were used as a probe. The immunogold nanoparticles specially react with the antibody against IgG(H)[anti IgG(H)] which was immobilzed on the quartz substrates. The identification between the immunogold colloids and the anti IgG(H) was examined by surface enhanced Raman specta(SERS) of MBA after and before silver staining and confirmed by UV-Vis absorption spectra.

Key words: Surface enhanced Raman spectroscopy(SERS), Nanoparticle, Immunization, Labeling

CLC Number:

O644.1
Q511

TrendMD:

XU Shu-Ping, WANG Lian-Ying, XU Wei-Qing, ZHAO Bing, YUAN Hang, MA Lan, BAI Yu-Bai, FAN Yu-Guo. Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining[J]. Chem. J. Chinese Universities, 2003, 24(5): 900.

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Immunological Identification with SERS-labeled Immunogold Nanoparticles by Silver Staining

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