Cholesterol Biosensors Based on Cholesterol Oxidase Immobilized in a Silica Sol-gel Matrix on a Prussian Blue Modified Electrode

Abstract

Abstract: Acholesterol biosensor prepared by immobilization of cholesterol oxidase in a silica sol-gel matrix on the surface of Prussian Blue-modified glass carbon electrode was reported. The properties of the sensor were investigated. It is based on the detection of hydrogen peroxide produced by ChOx at 0. 0-0. 1 V. The measurements conditions were optimized. Cholesterol can be determined in the concentration range of 5 × 10^-7～8 × 10^-5mol/Lby voltammetry or 5 ×10^-6～5 × 10^-4mol/Lby amperometry. The method gives a high sensitivity owing to the electrocatalytic layer of Prussian Blue to the electro-reduction of enzymatic production of hydrogen peroxide. In addition, the effect of easily oxidizable substances presented in samples such as ascorbic acid and uric acid can be minimized.

Key words: Biosensor, Cholesterol oxidase, Prussian Blue, Sol-gel, Electrode

CLC Number:

O657

TrendMD:

LI Jian-Ping, PENG Tu-Zhi. Cholesterol Biosensors Based on Cholesterol Oxidase Immobilized in a Silica Sol-gel Matrix on a Prussian Blue Modified Electrode[J]. Chem. J. Chinese Universities, 2003, 24(5): 798.

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Cholesterol Biosensors Based on Cholesterol Oxidase Immobilized in a Silica Sol-gel Matrix on a Prussian Blue Modified Electrode

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