HPLC and ESI-TOF-MS Analysis of the Anomeric Isomers of Morroniside

Abstract

Abstract: Morroniside consists of two anomeric isomers, α-morroniside and β-morroniside.These two anomeric compounds have been separated by reversed-phase high-performance liquid chromatography(HPLC) using a gradient mobile phase of acetonitrile-water at 7 ℃, and verified to be anomers of morroniside by ESI-TOF-MS.The higher peak in the chromatogram was appointed to α-morroniside according to NMR data reported in previous literature.The molar ratio of α-morroniside to β-morroniside was found to be 2.855∶1 at 25℃. Temperature appeared to be an important factor, which affects the separation result, and a better separation was achieved at a lower temperature.The relative peak height of β-morroniside in the total ion current chromatogram measured with positive ion ESI-TOFmass spectrometer was only a half of that in UVchromatogram.This indicates that β-morroniside is less sensitive to positive ion electrospray ionization than the α-isomer.

Key words: Morroniside, Anomers, ESI-MS

CLC Number:

O657.63

TrendMD:

YAN Cun-Yu, ZHAO Yi-Min, QIAO Shan-Yi, LIU Qin, XIE Jian-Wei, ZHANG Yong-Xiang . HPLC and ESI-TOF-MS Analysis of the Anomeric Isomers of Morroniside[J]. Chem. J. Chinese Universities, 2003, 24(10): 1784.

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HPLC and ESI-TOF-MS Analysis of the Anomeric Isomers of Morroniside

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