Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (10): 1787.

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Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe

LI Wen-You¹, WU Hui-Ling¹, HE Xi-Wen¹, LIANG Hong²

1. Department of Chemistry, State Key Laboratory of Elemento-organic Chemistry, Nankai University, Tianjin 300071, China;
2. Department of Chemistry, Guangxi NormaLUniversity, Guilin 541004, China

Received:2002-03-20 Online:2003-10-24 Published:2003-10-24

Abstract

Abstract: The fluorescence spectra and binding reaction of neutral red with nucleic acids have been studied.At pH=4.5, a new method for the determination of trace nucleic acids based on fluorescence quenching measurement was established.When 8.0×10^-6mol/L NRis employed, the linear ranges of the calibration graphs are 0.08-2.0 μg/mLfor calf thymus DNA(ctDNA), 0.12-2.2 μg/mLfor fish sperm DNA(fsDNA) and 0.30-1.4 μg/mLfor yeast RNA(yRNA), respectively.The corresponding detection limits are 30, 33 and 66ng/mL, respectively.The real samples were analyzed satisfactorily.

Key words: Neutral red, Nucleic acids, Fluorescence analysis

CLC Number:

O657

TrendMD:

LI Wen-You, WU Hui-Ling, HE Xi-Wen, LIANG Hong . Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe[J]. Chem. J. Chinese Universities, 2003, 24(10): 1787.

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Determination of Trace Nucleic Acids Using Neutral Red as the Fluorescent Probe

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