Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (10): 1770.

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Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay

CHEN Shou-Hui^1,2, CUI Xiao-Qiang¹, YANG Fan¹, NI Yong-Nian², YANG Xiu-Rong¹

1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academyof Sciences, Changchun 130022, China;
2. Department of Chemistry, Nanchang University, Nanchang 330047, China

Received:2002-11-05 Online:2003-10-24 Published:2003-10-24

Abstract

Abstract: It is impossible for surface plasmon resonance to measure directly the binding kinetics between a low-molecular-weight analyte interacting and its immobilized binding partner.Solution competition method was applied to the kinetic study of the interaction between morphine and its antibody.The affinity constant between the antibody of morphine and morphine-BSAimmobilized on the sensor chip was also obtained.The result showed that the affinity of polyclonal antibody is stronger than that of monoclonal antibody.And it also indicated that the protein combined with the analyte affected the binding of antibody to antigen.

Key words: Surface plasmon resonance(SPR), Kinetics, Morphine, Solution competition method, Immunoassay

CLC Number:

O657

TrendMD:

CHEN Shou-Hui, CUI Xiao-Qiang, YANG Fan, NI Yong-Nian, YANG Xiu-Rong . Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay[J]. Chem. J. Chinese Universities, 2003, 24(10): 1770.

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Kinetic Characteristics of the Interaction Between Morphine and Its Antibody by SPR Competitive Immunoassay

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