Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (9): 1684.

• Articles • Previous Articles     Next Articles

Synthesis, Characterization and Evaluation of a Novel Ester-bonded for Reversed-phase HPLC

HUANG Xiao-Jia, LIU Xue-Liang, WANG Jun-De, CONG Run-Zi   

  1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China
  • Received:2001-05-22 Online:2002-09-24 Published:2002-09-24

Abstract: Anovel ester bonded packing for reversed phase HPLC(RP HPLC) was synthesized by reacting octanoic acid with β (3,4 epoxycyclohexyl) ethyltrimethoxy silane and then the intermediate product was coupled onto porous silica. Characterization of the prepared packing was carried out with elemental analysis, solid state 13 C NMR, and Fourier transform infrared spectrometry. Hydrophobicity, selectivity and silanophilic activity of the packing were evaluated by using organic components including acidic, basic and neutral analytes as probe molecules and methanol water as binary mobile phase. The usage ranges of pHand stability of the packing were also evaluated. The results show that the novel stationary phase has excellent chromatographic properties and resist to hydrolysis between pH=2.5-7.5, it can be used for the separation of basic solutes efficiently.

Key words: Silane coupling agent, High performance liquid chromatography, Reversed phase packing, Ester bonded stationary phase

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