Studies on the Solution Conformations of Bt Toxic CryⅠ Ab Expressed by Bt Transgenic Rice

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (6): 1081.

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Studies on the Solution Conformations of Bt Toxic CryⅠ Ab Expressed by Bt Transgenic Rice

WU Zhi-Ping^1,2, XU Bu-Jin², SHU Qing-Yao², GONG Li-Qin³, YU Hong⁴, YE Gong-Yin⁴, CHEN Zi-Yuan²

1. Department of Chemistry, Hangzhou 310029, China;
2. Institute of Nuclear Agricultural Sciences, Hangzhou 310029, China;
3. Department of Biology, Hangzhou 310029, China;
4. Department of Plan Protection, Zhejiang University, Hangzhou 310029, China

Received:2001-03-12 Online:2002-06-24 Published:2002-06-24

Abstract

Abstract: The conformations of the toxic protein CryⅠAb in its solution were studied by fluorescence. The results showed that the toxic protein fluorescence intensity is improved with its pHvalue increase and reaches its maximum at pHequivalent to 11. Adding some organic solvents such as methanol, ethanol, isopropanol result in the emission wavelength of fluorescence of the toxic protein blue shift and its fluorescence intensity increase. However, the toxic protein fluorescence emission wavelength no long blue shifts as these organic solvents concentration beyond certain values. Acetone can quench all fluorescence of the toxic protein CryⅠAb and acrylamide can quench 86% of the total fluorescence of the toxic protein, but KIcan′t quench any fluorescence. It implicates that the most of tryptophan residuals of CryⅠAb are located on the toxic protein surface where the negative charge is rich.

Key words: Transgenic rice, Bt toxic protein CryⅠAb, Conformation

CLC Number:

O629.73

TrendMD:

WU Zhi-Ping, XU Bu-Jin, SHU Qing-Yao, GONG Li-Qin, YU Hong, YE Gong-Yin, CHEN Zi-Yuan . Studies on the Solution Conformations of Bt Toxic CryⅠ Ab Expressed by Bt Transgenic Rice[J]. Chem. J. Chinese Universities, 2002, 23(6): 1081.

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Studies on the Solution Conformations of Bt Toxic CryⅠ Ab Expressed by Bt Transgenic Rice

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