Langmuir Aggregation of Chromophore in Protein Macromoleculeand Its Application──Interaction of Phloxine B with Proteins

Abstract

Abstract: The microphase adsorption-spectral correction(MASC) technique was applied to investigation ofthe interaction of phloxine B(PB) with proteins.The aggregation of PBobeys the Langmuir monolayeradsorption and it has been used in the determination of protein solution at pH3.8.The results show thatthe adsorption ratios of PBto proteins are n(PB)∶ n(BSA) = 1:155, n(PB):n(OVA) = 1:58, n(PB):n(Mb) = 1:18, their adsorption constants are 2.17 × 10⁴, 1.18 × 10⁴, 3.25× 105 and their real molarabsorptivities ε_BSA-PB^555nm=7.94 × 10⁶, ε_OVA-PB^555nm=3.39× 10⁶, ε_Mb-PB^555nm= 1.21 × 10⁶ L ·mol^-1 ·cm^-1.The interac-tion of PBwith proteins is sensitive and selective in the presence of EDTA.For analysis of samples, the re-covery of protein is between 96.7% and 107% and the relative standard deviation 4.6%.

Key words: MASC technique, Protein, Langmuir aggregation, Phloxine B(PB)

CLC Number:

O657.3

TrendMD:

GAO Hong-Wen, CHEN Yun-Sheng. Langmuir Aggregation of Chromophore in Protein Macromoleculeand Its Application──Interaction of Phloxine B with Proteins[J]. Chem. J. Chinese Universities, 2002, 23(5): 825.

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Langmuir Aggregation of Chromophore in Protein Macromoleculeand Its Application──Interaction of Phloxine B with Proteins

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