Synthesis and Characterization of Double-tailed Phosphate Surfactant and Extraction of Protein with Its Reversed Micelles

Abstract

Abstract: A new phosphate surfactant that has never been reported before, sodium di-ethyloleyl phosphate acid(DEOPA), which fits for the extraction of proteins with reversed micellar system, has been synthesized. Its structure has been characterized with ¹H NMR and FTIR spectrum. Its surfacial physico-chemical properties, such as cmc(Critical Micellar Concentration), HLB(Hydrophilic-Lipophilic Balance), and its solubilization effect to water was detected. Extraction of proteins using reverse micelles composed of DEOPA and isooctane was carried out. The results show that DEOPA is of relatively high surfactant activity, which means it fits for composing reversed micellar systems, and is of superiority when extracting large molecular weight proteins.

Key words: Double-tailed phosphate surfactant, Synthesis, Reversed micelle, Extraction, Protein

CLC Number:

O647

TrendMD:

YAN Yun, CHU Ying, LIN Yi, XING Shuang-Xi, LIU Ying-Ying, LIU Jing-Lin, WU Zi-Sheng . Synthesis and Characterization of Double-tailed Phosphate Surfactant and Extraction of Protein with Its Reversed Micelles[J]. Chem. J. Chinese Universities, 2002, 23(10): 1921.

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Synthesis and Characterization of Double-tailed Phosphate Surfactant and Extraction of Protein with Its Reversed Micelles

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