A CD and FT-Raman Study on the Secondary Structure of Saporin-S6

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (7): 1117.

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A CD and FT-Raman Study on the Secondary Structure of Saporin-S6

WANG Yong-Ting¹, HUANG Pin-Wei², LI Zheng-Qiang², XU Fei¹, MENG Qing-Fan¹, YANG Yi¹, SHI Xue-Hui¹, LI Qing-Shan¹

1. College of Life Science;
2. Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China

Received:2000-05-12 Online:2001-07-24 Published:2001-07-24

Abstract

Abstract: Saporin-S6 is purified from the seeds of Saponaria officinalis and its CDand FT-Raman spectra are determined. From the CDspectra of fresh protein aqueous and the same aqueous that was laid aside for 90 days, it is proved that_saporin S₆ has unusual stability. Throughout the FT-Raman spectra of saporin-S6, we work out that the α-helix of saporin-S6 is 26%, while β-fold is 37%, and random coil is 37%.

Key words: Saporin, Secondary structure, Raman spectra, Circular dichroism

CLC Number:

O629.73

TrendMD:

WANG Yong-Ting, HUANG Pin-Wei, LI Zheng-Qiang, XU Fei, MENG Qing-Fan, YANG Yi, SHI Xue-Hui, LI Qing-Shan . A CD and FT-Raman Study on the Secondary Structure of Saporin-S6[J]. Chem. J. Chinese Universities, 2001, 22(7): 1117.

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A CD and FT-Raman Study on the Secondary Structure of Saporin-S6

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