Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (8): 1183.

• Articles • Previous Articles     Next Articles

Separation of Proteins by Microemulsion Electrokinetic Chromatography

ZHANG Xuan, HU Ya-Nan, WANG Yi-Ming, ZHOU Guo-Hua, LUO Guo-An   

  1. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:1999-09-28 Online:2000-08-24 Published:2000-08-24

Abstract: Microemulsion electrokinetic chromatography(MEEKC) is a new technique which was employed to separate both basic and acidic proteins simultaneously. Optimum separation conditions were investigated with respect to ionic strength and the composition of microemulsion. The results show that if a stable microemulsion formed, the efficiency of protein separation was slightly affected by the concentration of the background and the composition of microemulsion. The fundamental characteristics of MEEKCwere studied in comparison with micellar electrokinetic chromatography(MEKC). The mechanism of MEEKCwas also investigated. The results show that MEEKCis an alternative way for protein separations with a high efficiency.

Key words: Microemulsion electrokinetic chromatography, Micellar electrokinetic chromatography, Protein

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