Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (6): 873.

• Articles • Previous Articles     Next Articles

An Electrochemiluminescence Immunosensor for Determination of Methsulfuron-methyl

XING Wan-Li1, CHAO Fu-Huan1, JIANG Zhong-Hua2, MA Li-Ren2   

  1. 1. Institute of Hygiene and Environmental Medicine, Tianjin 300050, China;
    2. Institute of Radiation Medicine, Beijing 100850, China
  • Received:1999-10-15 Online:2000-06-24 Published:2000-06-24

Abstract: An electrochemiluminescence(ECL) biosensor based on solid-phase antigen competitive immunoreactions was constructed to determine methsulfuron-methyl using Ru(bpy)32+ as labeling reagent. The coating hapten was bound to the surface of microparticles through avitin-biotin reaction. An electrochemical flow cell containing working electrodes and counter electrodes for initiation of the ECLreaction was constructed. Results showed that the detection limit for methsulfuron-methyl was 0.1 ng/mL.

Key words: Electrochemiluminescence, Immunosensor, Methsulfuron-methyl

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