Abstract: Amixed functional stationary phase containing aza crown ether and cyclodextrin for highperformance liquid chromatography was prepared via a continuous solidliquid reaction method. The new mixed functional stationary phase was used to separate some disubstituted benzene isomers such as nitrophenols, nitroanilines and phthalic acids. The results show that the separation ability of the new mixed functional stationary phase for the disubstituted benzene isomers is better than that on the aza crown ether bonded silica and cyclodextrin bonded silica stationary phases. Chiral separations of glycine derivatives, PMGly, MPGly and BPGly, were partially achieved on the new mixed functional stationary phase with phosphate buffer solution as the mobile phase.

Key words: HPLC, Crown ether, Cyclodextrin, Mixed functional bonded silica stationary phase