Observation of the Different Binding Rates of Δ-and Λ-[Ru(bpy)2(HPIP)]2+with Calf Thymus DNA via CD Spectra

Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (4): 523.

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Observation of the Different Binding Rates of Δ-and Λ-[Ru(bpy)₂(HPIP)]²⁺with Calf Thymus DNA via CD Spectra

LIU Jin-Gang¹, YE Bao-Hui¹, JI Liang-Nian¹, TIAN Xuan², FU Yao-Hong³

1. Department of Chemistry, Zhongshan University, Guangzhou, 510275;
2. State Key Laboratory, of Applied Organic Chemistry, Lanzhou University, Lanzhou, 730000;
3. State Key LaboLaboratory of Bio-Organic and Natural Products, Shang hai Institute of Organic Chemistry, Shanghai, 200032

Received:1999-02-01 Online:1999-04-24 Published:1999-04-24

Abstract

Abstract: The complexes of Δ-and Λ-[Ru(bpy)₂(HPIP)]²⁺(bpy=2,2'-bipyridine, HPIP=2-(2-hydroxyphenyl)imidazo [4,5-f][1,10]phenanthroline) were prepared and their racemic complex were dialysed against calf thymus DNA(CT-DNA). During the course of dialysis with the solution stirring for 48h, the CD signals of the dialyzate experience disappearing-appearing-disappearing. However, in the similar conditions except for not stirring, strong CD signals appeared. From the results obtained, several novel opinions were suggested. Firstly, the evidence of different binding rates of Δ-andΛ-enantiomers of polypyridyl ruthenium(Ⅱ) complexes with CT-DNA, the first example, was demonstrated. As for the dialysis experiment, binding of -enantiomer is dynamically preferred for CT-DNA. Secondly, the different binding rates may be deduced from different binding modes of the enantiomers.

Key words: Ruthenium(Ⅱ) complexes, DNA, Enantiomers, CD spectra, Binding rate

CLC Number:

O614.92

TrendMD:

LIU Jin-Gang, YE Bao-Hui, JI Liang-Nian, TIAN Xuan, FU Yao-Hong . Observation of the Different Binding Rates of Δ-and Λ-[Ru(bpy)₂(HPIP)]²⁺with Calf Thymus DNA via CD Spectra[J]. Chem. J. Chinese Universities, 1999, 20(4): 523.

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Observation of the Different Binding Rates of Δ-and Λ-[Ru(bpy)₂(HPIP)]²⁺with Calf Thymus DNA via CD Spectra

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