Purif ication and Characterization of L-Amino Acid Oxidase from Agkist rodon Blomhof fii Ussurensis Snake Venom of Changbai Mountains

Abstract

Abstract: The L-a.a.oxidase of Agkist rodon blom hoffii ussurensis of Changbai Mountains in northeast of China has been separated by using ion-exchange and gel filtration techniques.This enzyme is composed of two subunits, The molecular weight of one subunit is about 36000, The another is about 57000, determined by sodium dodecyl sulfate-polyacryamide gelelectrophoresis and mat rix assisted laser desorptionion/time of flight mass spect rometry.The activity of L-a.a.oxidase determined using L-Leu as substrate.The optimal pHof the enzyme is 4.55and 89.The UV-Visible absorption spectrum of L-a.a.oxidase shows the characteristics of flavorpro-teins.

Key words: A gkist rodon blom hoffii ussurensis snake venom, L-Amino acid oxidase, Purifica-tion, Characterization

CLC Number:

O656.2

TrendMD:

SUN Ming-Zhong, DING Lan, ZHAO Da-Qing, NI Jia-Zua . Purif ication and Characterization of L-Amino Acid Oxidase from Agkist rodon Blomhof fii Ussurensis Snake Venom of Changbai Mountains[J]. Chem. J. Chinese Universities, 1999, 20(1): 37.

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Purif ication and Characterization of L-Amino Acid Oxidase from Agkist rodon Blomhof fii Ussurensis Snake Venom of Changbai Mountains

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