Spectroscopic Studies on the Binding of Lanthanides to Apoovotransferrin

Abstract

Abstract: The binding of Eu³⁺to chicken egg apoovotransferrin has been studied by monitoring the change of differential UVspectra at 245 nm. Conditional equilibrium constants for the complexation of Eu³⁺with chicken egg apoovotransferrin in 0.1 mol/Lhepes, pH7.4, at 25 ℃ have been measured. The results are lgK₁=8.21±0.20 and lgK₂=4.60±0.11 for complexation of Eu³⁺. The molar absorptivity per binding site for Eu³⁺is (2.1±0.1)×10⁴cm^-1·L·mol^-1. The titration of N-terminal monoferric ovotransferrin with Eu³⁺(differential UVspectra) and Tb³⁺(fluorescence) indicate that the binding of lanthanide ions at N-terminal binding site is stronger than that at C-terminal binding site.

Key words: Ovotransferrin, Lanthanide ions, Spectra

CLC Number:

O657.3

TrendMD:

YANG Bin-Sheng, Wesley R. Harris . Spectroscopic Studies on the Binding of Lanthanides to Apoovotransferrin[J]. Chem. J. Chinese Universities, 1998, 19(7): 1057.

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Spectroscopic Studies on the Binding of Lanthanides to Apoovotransferrin

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