On-line Capillary Electrophoresis-mass Spectrometry for Determination of Peptide Mixture and Protein Mixture

Abstract

Abstract: The influence of uncoated, coated linear polyacrylamide(LPA) and amino coated capillaries on the separation of five peptide mixtures, including angiotensin Ⅰ, Ⅱ, Ⅲ, substance P and somatostain, was studied. The results showed that the peptide mixtures could not be separated completely in uncoated capillary by volatile buffer which was suitable for the MSanalysis, whereas they could be separated in LPA and amino coated capillary by pH 5.0 and pH 4.5 NH₄Ac buffer respectively. In addition, the above peptide mixtures, protein mixtures comprised of myoglobin and carbonic anhydrase Ⅱ, tryptic digests of erythropoietin, were separated and characterized by capillary electrophoresis mass spectrometry(CE-ESI-MS) with electrospray ionization interface. The factors affecting the determination in CE-ESI-MS were also discussed.

Key words: Capillary electrophoresis-mass spectrometry, Coated capillary, Peptide, Protein, Tryptic mapping of erythropoietin

CLC Number:

O657.8

TrendMD:

ZHOU Guo-Hua, LUO Guo-An, ZHOU Ke-Yu, GU Zhuo-Liang. On-line Capillary Electrophoresis-mass Spectrometry for Determination of Peptide Mixture and Protein Mixture[J]. Chem. J. Chinese Universities, 1998, 19(3): 353.

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On-line Capillary Electrophoresis-mass Spectrometry for Determination of Peptide Mixture and Protein Mixture

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