Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography

Abstract

Abstract: The synthesis of packing of oxygen-ethane as end-ligand bonded to silica andseparation for recombinant human interferons(rhIFN) in high-performance hydrophobicinteraction chromatography has been studied in this paper. It was showed that the packingsynthesized was not only suitable for separating standard proteins, but also for different rhIFNs,i. e., rhIFN-α_Aexpressed in yeast, rhIFN-α_Aand rh1FN-γ in E. colt. The purify of threerhIFNs purified by one-step HPHIC, which rhIFN-γ expressed in E. colt was recognized as80%, rhIFN-α_Aexpressed in yeast as 70%, and rhIFN-α_Aexpressed in E. colt as 30%, wasmore than that of other chromatography besides affinity chromatography.

Key words: High-performance hydrophobic interaction chromatography, Purification, Recombinant human interferon

CLC Number:

O657.7

TrendMD:

GUO Li-An, ZHU Bao-Quan. Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography[J]. Chem. J. Chinese Universities, 1997, 18(9): 1462.

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Synthesis of High Efficiency Packing and Using for Recombinant Human Interferons Separation in High-performance Hydrophobic Interaction Chromatography

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