3,3',5,5'-Tetramethylbenzidine(TMB)-H2O2-HRP Voltammetric Immunoassay for the Determination of IgE in Human Serum

Abstract

Abstract: Adifferential pulse voltammetric immunoassay associated with the TMB-H₂O₂^-HRPfor the determination of IgEantibody was developed. The enzymatic reaction product produced by HRPcatalyzing the oxidation of H₂O₂ with TMBexhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH 4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgEantibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISAprocedure. It has been ap-plied to the determination of IgEin human serum with satisfactory results.

Key words: 3,3',5,5'-Tetramethylbenzidine (TMB), IgE, ELISA, Differential pulse voltammetry

CLC Number:

O657.14

TrendMD:

HE Ya-Nan, CHEN Hong-Yuan . 3,3',5,5'-Tetramethylbenzidine(TMB)-H₂O₂^-HRP Voltammetric Immunoassay for the Determination of IgE in Human Serum[J]. Chem. J. Chinese Universities, 1997, 18(8): 1306.

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3,3',5,5'-Tetramethylbenzidine(TMB)-H₂O₂^-HRP Voltammetric Immunoassay for the Determination of IgE in Human Serum

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