Synthesis and Evaluation of Cy7-Rituximab Targeting CD20 Antigen for in vivo Animal Fluorescence Imaging

doi:10.7503/cjcu20130368

Abstract

Abstract:

A new fluorescence molecular imaging probe Cy7-Rituximab was synthesized through the connection of fluorescence Cy7 dye and protein Rituximab. The probe was fully characterized by fluorescence spectrophotometer, SDS-PAGE and confocal microscopy. The uptake of Cy7-Rituximab probe in DLBCL cells was observed by laser scanning confocal microscope. Cy7-Rituximab probe of 0.4 mL(0.6 mg/mL) was injected to BALB/C nude mice through tail vein. The biodistribution of the novel probe Cy7-Rituximab was eventually observed, especially its specific concentration in spleen.

Key words: Optical imaging probe, Living imaging, CD20 antigen, Rituximab

CLC Number:

O629
O652

TrendMD:

LIN Xin-Feng, ZHU Hua, HONG Ye, YANG Zhi. Synthesis and Evaluation of Cy7-Rituximab Targeting CD20 Antigen for in vivo Animal Fluorescence Imaging[J]. Chem. J. Chinese Universities, 2013, 34(9): 2139.

References

[1] Weissleder R., Science, 2006, 312, 1168—1171

[2] Herschman H. R., Science, 2003, 302, 605—608

[3] Weissleder R., Molecular Imaging Radiology, 1999, 212, 609—614

[4] Danthi S. N., Pandit S. D., Li K. C. P., Acad. Radiol., 2004, 11, 1047—1105

[5] Bakker W. H., Albert R., Bruns C., Breeman W. A. P., Hofland L. J., Marbach P., Pless J., Pralet D., Stolz B., Koper J. W., Lamberts S. W. J., Visser T. J., Krenning E. P., Life Sci., 1991, 49, 1583—1591

[6] Thakur M., Kolan H., Rifat S., Li J., Rux A., John E., Halmos G., Schally A., Int. J. Oncol., 1996, 9, 445—451

[7] Sui B. S., Wu E. H., Chen Y. B., Magnetic Resonance Imaging Diagnostics, People's Medical Publishing House, Beijing, 1994, 62—68(隋邦森, 吴恩惠, 陈雁冰. 磁共振诊断学, 北京: 人民卫生出版社, 1994, 62—68)

[8] Liu F., Wu T., Hu M. M., Peng X. J., Fan J. L., Chem. J. Chinese Universities, 2012, 33(10), 2239—2243(刘飞, 吴彤, 胡明明, 彭孝军, 樊江莉. 高等学校化学学报, 2012, 33(10), 2239—2243)

[9] Corrie L., Nature, 2001, 412, 372—374

[10] He W. N., Tu X. J., Guo X. Q., Chem. J. Chinese Universities, 2012, 33(11), 2411—2416(何微娜, 涂熹娟, 郭祥群. 高等学校化学学报, 2012, 33(11), 2411—2416)

[11] Benson R. C., Kues H. A., J. Chem. Eng. Data, 1977, 22, 379—383

[12] Jacques S. L., Phys. Med. Biol., 2013, 58, 37—61

[13] Kim T. I., Park J., Park S., Choi Y., Kim Y., Chem. Commun.(Camb), 2011, 47, 12640—12642

[14] Golay J. T., Clark E. A. Beverley P. C., J. Immunol., 1985, 135, 3795—3801

[15] Stashenko P., Nsdler L. M., Hardy R., Schlossman S. F., J. Immunol., 1980, 125, 1678—1685

[16] Nadler L. M., Karsmeyer S. J., Anderson K. C., Boyd A. W., Slaughenhoupt B., Park E., Jensen J., Coral F., Mayer R. J., Sallan S. E., Ritz J., Schlossman S. F., J. Clin. Invest., 1984, 74, 332—340

[17] Stashenko P., Nsdler L. M., Hardy R., Schlossman S. F., Proc. Natl. Acad. Sci. USA, 1981, 78, 3848—3852

[18] Perz J., Topaly J., Fruehauf S., Hensel M., Ho A. D., Leuk Lymphoma, 2002, 43, 149—151

[19] Einleld D. A., Brown J. P., Valentine M. A., Clark E. A., Ledbetter J. A., EMBO J., 1988, 7, 711—717

[20] Press O. W., Appelbaum F., Ledbetter J. A., Martin P. J., Zarling J., Kidd P., Thomas E. D., Blood, 1987, 69, 584—591

[21] Anderson K. C., Bates M. P., Slaughenhoupt B. L., Pinkus G. S., Schlossman S. F., Nadler L.M., Blood, 1984, 63, 1424—1433

[22] Wu Y., Cai W. B., Chen X. Y., Mol. Imaging Biol., 2006, 8, 226—236

[23] Greg T. H., Bioconjugate Techniques, Academic Press, San Diego, 2008, 396—405

[24] Wang X. J., Yang Z., Lin B. H., Chin. J. Nucl. Med., 2006, 26, 226—230(王雪鹃, 杨志, 林保和. 中华核医学杂志, 2006, 26, 226—230)

[25] Arutselvan N., Gayatri G., Carsten H. N., Mol. Imaging Biol., 2012, 14, 608—616

[26] Kirschenbaum D. M., Anal. Biochem., 1975, 68, 465—484

[27] Liu Y., Li Y. F., Zheng K. Y., Fei X. F., Chem. Res. Chinese Universities, 2012, 28(3), 449—453

[28] Kussmann M., Nordhoff E., Rahbek-Nielsen H., Haebel S., Rossel-Larsen M., Jakobsen L., Gobom J., Mirgorodskaya E., Kroll-Kristensen A., Palmll L., Roepstorff P., J. Mass Spectrom., 1997, 32, 593—601

[29] Javier F. M., Jeremy P., Matthew D. S., J. Cell Biol., 2012, 196, 419—434