Protection of Myocardial Mitochondria Against the Active Oxygen Injury by Horseradish Peroxidase

Abstract

Abstract: The protection of myocardial mitochondria against the free radical injury byhorseradish peroxidase was studied. The free radical injury model of mitochondria in vitrowas set up. Horseradish peroxidase could inhibit the generation of free radicals and lipidperoxides, keep cytochrome oxidase of mitochondria membrane with a high activity, and makethe mitochondria remain relative intact in structure. The results suggest that horseradishperoxidase as peroxide scarvenger could decrease the free radical injury of mitochondria.

Key words: Horseradish peroxidase, Myocardial mitochondria, Free radicals

CLC Number:

Q554

TrendMD:

XING Hui, FENG Yan, GAO Gui, CHEN Si-Geng, YANG Tong-Shu. Protection of Myocardial Mitochondria Against the Active Oxygen Injury by Horseradish Peroxidase[J]. Chem. J. Chinese Universities, 1997, 18(10): 1628.

[1]	ZHU Haotian, JIN Meixiu, TANG Wensi, SU Fang, LI Yangguang. Properties of Transition Metal-biimidazole-Dawson-type Tungstophosphate Hybrid Compounds as Supports for Enzyme Immobilization [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220328.
[2]	GAO Xia,PAN Huibin,QIAO Chengfang,CHEN Fengying,ZHOU Yuan,YANG Wenhua. Construction of HRP Immobilized Enzyme Reactor Based on Hierarchically Porous Metal-organic Framework and Its Dye Degradation Application^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1591.
[3]	TENG Yu,YANG Shaoming,BAI Chaopeng,ZHANG Jian. Preparation of HRP Molecularly Imprinted Electrochemical Sensor with Multi-walled Carbon Nanotubes as Sensitizing Materials and the Detection of H₂O₂ ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 78.
[4]	FAN Hongliang, ZHANG Tao, JIN Wei, JIN Qinhan. Novel Label-free Assay of Telomerase Based on DNAzyme^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 625.
[5]	ZHOU Haifeng, YANG Dongjie, QIU Xueqing, WU Xiaolei. Structural Characterization, Adsorption and Dispersion Properties of Sodium Lignosulfonate by Horseradish Peroxidase Incubation^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 895.
[6]	QU Jian-Ying, KANG Shi-Ping, LOU Tong-Fang, DU Xue-Ping. Detection of H₂O₂ at Composite Membrane of [bmim] FeCl₄ and PVA Loading HRP Modified Graphene/nano Au [J]. Chem. J. Chinese Universities, 2013, 34(9): 2097.
[7]	LU Hai-Xia, WU Zai-Sheng, JIN Xiao-Yong, SHEN Guo-Li^*, YU Ru-Qin. Novel Electrochemical Sensor Based on Seminetwork-type Au Nanoparticles Labled Horseradish Peroxidase [J]. Chem. J. Chinese Universities, 2009, 30(2): 263.
[8]	JIANG Zhi-Liang^*, LI Jian-Fu, LIANG Ai-Hui, LI Ji-Shun, TANG Ya-Fang, WANG Su-Mei, ZHANG Nan-Nan. Rayleigh Scattering Spectral Determination of Ultratrace Horseradish Peroxidase Based on o-Phenylenediamine Particles and Its Application [J]. Chem. J. Chinese Universities, 2008, 29(10): 1973.
[9]	FENG Xia-Guang, ZHANG Min, ZHAO Hu, WANG Huai-You. Determination of Vancomycin in Injection via* Enzyme Catalysis-Fluorescence Quenching Method [J]. Chem. J. Chinese Universities, 2007, 28(7): 1270.
[10]	ZHOU Jun^1,3, WU Ke², SUN Yue³, CONG Jian-Bo², WANG Chang-Zhen², CHANG Xiang³, XIAN Hong², ZHU Yong-Fa¹*. Development of a Quantitative Method for Analyzing Gas-phase Free Radicals in Mainstream Cigarette Smoke [J]. Chem. J. Chinese Universities, 2007, 28(10): 1846.
[11]	WANG Jiao-Ning, REN Ji-Cun. Determination of Horseradish Peroxidase by Capillary Electrophoresis with Flow-injection Chemiluminescence Detection and Its Application in Immunoassay [J]. Chem. J. Chinese Universities, 2004, 25(S1): 171.
[12]	LIU Bing, YANG Ming-Hui, YANG Hai-Feng, SHEN Guo-Li, YU Ru-Qin . A Biosensor for Hydrogen Peroxide Based on Peroxidase Immobilized by Plasma Polymerized Film [J]. Chem. J. Chinese Universities, 2004, 25(10): 1820.
[13]	LI Wen-Jiang, SONG Li-Zhu, SUN Chang-Qing, WANG Zi-Chen, GUO Yu-Peng, ZHAO Chun, Xu Ying-kai. Preparation of Ultrathin Multilayer Films Containing Poly(4-vinylpyridine) Complex of Osmium and Horseradish Peroxidase and Its Electrochemical Behavior [J]. Chem. J. Chinese Universities, 2001, 22(9): 1601.
[14]	JIANG Jun-Guang, WANG Zhen-Xin, LIU Chang-Wei, LIU Dian-Jun, YANG Xiu-Rong, DONG Shao-Jun . Investigation of Thermal Unfolding Process of Cyanic Adduct of Horseradish Peroxidase by Fourier Transform Infrared and Circular Dichroism Spectrometry [J]. Chem. J. Chinese Universities, 2001, 22(7): 1131.
[15]	ZHANG Shu-Sheng, JIAO Kui, CHEN Hong-Yuan, WANG Man-Xia, ZHANG Cheng-Liang, ZHANG Zuo-Fang . MAP-H₂O₂-HRP Voltammetric Enzyme linked Immunoassay for Trace Southern Bean Mosaic Virus [J]. Chem. J. Chinese Universities, 2000, 21(8): 1200.

Protection of Myocardial Mitochondria Against the Active Oxygen Injury by Horseradish Peroxidase

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments