Studies on the Immobilization of glucose Isomerase and the Properties of the Immobilized Glucose Isomerase

Abstract

Abstract: In this paper we successfully immobilized bilayer glucose isomerase in porous ptrimethylamine-polystyrene(TMPS)beads by use of a new method of immobilization of enzyme──molecular-deposition.The effects of immobilizing time,the concentration of nature enzyme, the concentration of hexamethylene bis(trimethylammonium bromide), the concentration BCDwere investigated.The properties of this immobilized enzyme were also studied.The results obtained show that the activity and the protein loading of per weight bilayer immobilized enzyme are doubled as compared to monolayer immobilized enzyme.The activity of immobilized bilayer glucose isomerase is 1200 IU/g wet gel.

Key words: Glucose isomerase, Immobilization, Molecular deposition

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GE Yu-Bin, WANG Li-Ping, KONG Wei, ZHOU Hui, LI Wei, SHEN Jia-Cong. Studies on the Immobilization of glucose Isomerase and the Properties of the Immobilized Glucose Isomerase[J]. Chem. J. Chinese Universities, 1996, 17(5): 735.

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Studies on the Immobilization of glucose Isomerase and the Properties of the Immobilized Glucose Isomerase

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