Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (5): 702.

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Studies on Uric Acid Microbiosensoe

LIU Bao-Hong, DENG Jia-Qi   

  1. Department of Chemistry, Fudan University, Shanghai 200433
  • Received:1995-04-12 Online:1996-05-24 Published:1996-05-24

Abstract: An electrode for the determination of uric acid was prepared.It employed a kind of bacillus as the biocatalyst and an oxygen gas electrode as the basic electrode, The effect of the culture medium, the response character of the electrode and its kinetic mechanism are studied.At suitable pH, the uric acid concentration is linear in the range of 1.0×10-5~4.0 ×10-4mol/L, the apparent Michaelis constant Km is 2.8×10-4 mol/L, the activation energy is 47000J/mol, and the temperature coefficient is 1.8.

Key words: Bacillus, Uric acid, Microbiosensor

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