Determination of DNA by Use of in situ Photochemical Fluorescence Probe──Sodium 9,10-Anthraquinone-2-sulfonate as a Photochemical Fluorescence Probe

Chem. J. Chinese Universities ›› 1996, Vol. 17 ›› Issue (11): 1706.

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Determination of DNA by Use of in situ Photochemical Fluorescence Probe──Sodium 9,10-Anthraquinone-2-sulfonate as a Photochemical Fluorescence Probe

LI Wen-You, XU Jin-Gou, GUO Xiang-Qun, ZHU Qing-Zhi, ZHAO Yi-Bing

The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen, 361005

Received:1996-05-13 Online:1996-11-24 Published:1996-11-24

Abstract

Abstract: An in situ photochemical fluorescence probe method for the determination of DNAwith sodium 9,10-anthraquinone-2-sulfonate(AQS) as a photochemical fluorescence probe was developed for the first time.It was based on the conversion of AQSinto an intensively fluorescent product on irradiating with UVradiation.The photochemical reaction is decelerated by DNA.The determination can be carried out by measuring the fluorescence intensity at a fixed time.The calibration graph was linear in the range 0～80 ng/mL CT DNA(r=0.9991), the limit of detection was 3.2 ng/mL CT DNA(n=9).The kinetic behaviour of the photochemical reaction and the effects of some experiment conditions were investigated and discussed in detail.The results of absorption spectra experiments and competitive binding experiments suggest that the interaction between AQSand DNAmay be intercalative.

Key words: DNA, Photochemical fluorescence probe, Sodium 9,10-anthraquinone-2-sulfonate

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LI Wen-You, XU Jin-Gou, GUO Xiang-Qun, ZHU Qing-Zhi, ZHAO Yi-Bing. Determination of DNA by Use of in situ Photochemical Fluorescence Probe──Sodium 9,10-Anthraquinone-2-sulfonate as a Photochemical Fluorescence Probe[J]. Chem. J. Chinese Universities, 1996, 17(11): 1706.

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Determination of DNA by Use of in situ Photochemical Fluorescence Probe──Sodium 9,10-Anthraquinone-2-sulfonate as a Photochemical Fluorescence Probe

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