Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation

Abstract

Abstract: In this paper,the chemiluminescence reaction of brucine,strychnine and ephedrines in KMnO₄-H⁺-reductant system has been characterized with respect to their ability to act as a rapid and sensitive chemiluminescence determination system of the above alkaloids.This method has been connected with HPLC which fitted with a C₁₈ column for separation so as to determine these alkaloids in sample.The detection limits are 1.0×10^-10g/mLof brucine,1.0×10^-8g/mL of strychnine and 1.0×10^-5 g/mLof ephedrines, respectively.The relative standard deviation is less than 3% and recovery is 94%～101%.Since the dissolved oxygen did not affect the determination,this method provide advantages of wider linear range,high sensitivity and easy to operate.

Key words: HPLC, Chemiluminescence, potassium permanganate, Alkaloids

TrendMD:

ZHU Long, FENG Man-Liang, WAN Xiu-Qin, LU Jiu-Ru. Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation[J]. Chem. J. Chinese Universities, 1996, 17(11): 1693.

[1]	WANG Ruina, SUN Ruifen, ZHONG Tianhua, CHI Yuwu. Fabrication of a Dispersible Large-sized Graphene Quantum Dot Assemblies from Graphene Oxide and Its Electrogenerated Chemiluminescence Behaviors [J]. Chem. J. Chinese Universities, 2022, 43(8): 20220161.
[2]	DU Shunfu, WANG Wenjing, EL⁃SAYED El⁃Sayed M., SU Kongzhao, YUAN Daqiang, HONG Maochun. A Chemiluminescent Zirconocene Coordination Tetrahedron [J]. Chem. J. Chinese Universities, 2022, 43(1): 20210628.
[3]	WANG Bodong, PAN Meichen, ZHUO Ying. Construction of Electrochemiluminescence Sensing Interface Based on Silver Nanoclusters-Silica Nanoparticles and Biomolecular Recognition [J]. Chem. J. Chinese Universities, 2021, 42(11): 3519.
[4]	LIAO Ni, ZHANG Jieyuan, HUANG Ziyang, ZHAO Yanxi, CHAI Yaqin, YUAN Ruo, ZHUO Ying. Construction of High Efficiency Uric Acid Sensor Based on the co-Crystal Enhanced Electrochemiluminescence from 9,10-Diphenylanthracene-perylene Microcrystals [J]. Chem. J. Chinese Universities, 2020, 41(9): 1989.
[5]	PAN Mengyun, FENG Liuxing, LI Hongmei. Absolute Quantification of Haemoglobin in Whole Blood by Species-unspecific Isotope-dilution Liquid Chromatography-inductively Coupled Plasma Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 1983.
[6]	CAO Zhiyuan, SUN Hui, SU Bin. Electrochemiluminescence of Quantum Dots: Research Progress and Future Perspectives [J]. Chem. J. Chinese Universities, 2020, 41(9): 1945.
[7]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[8]	ZHANG Jingjing, JIN Rong, FANG Danjun, JIANG Dechen. Voltage Modulated Electrochemiluminescence for Highly Sensitive Detection [J]. Chem. J. Chinese Universities, 2020, 41(11): 2421.
[9]	ZHAO Mengxin, MENG Zhe, LI Heping, MA Zongqin, ZHAN Haijuan, LIU Wanyi. Photodegradation of Antibiotic in Environmental Water by Graphene Oxide Modulation Bismuth Molybdate Under Visible Light Irradiation [J]. Chem. J. Chinese Universities, 2020, 41(11): 2479.
[10]	ZHAO Huanxi,WANG Qiuying,SUN Xiuli,LI Xue,MIAO Rui,WU Dongxue,LIU Shuying,XIU Yang. Discrimination of Ginseng Origins and Identification of Ginsenoside Markers Based on HPLC-MS Combined with Multivariate Statistical Analysis^† [J]. Chem. J. Chinese Universities, 2019, 40(2): 246.
[11]	YUE Ling,LIU Yajun. Basic Theory and Critical Problems in the Trajectory Surface Hopping Simulations on the Chemiluminescent Reaction of Organic Peroxides^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2113.
[12]	MIAO Rui,WU Dongxue,WANG Qiuying,ZHAO Huanxi,LI Xue,XIU Yang,LIU Shuying. Rapid Separation of Ginsenosides Based on Multi-walled Carbon Nanotubes^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2178.
[13]	LI Nan, ZHAO Huanxi, LI Jing, WANG Nan, YU Bohao, YUE Hao, YU Shanshan. Transformation of Total Notoginsenosides by Recombinant Endocellulase Fpendo5A^† [J]. Chem. J. Chinese Universities, 2017, 38(12): 2185.
[14]	LI Xue, ZHAO Huanxi, MIAO Rui, LI Wenying, XIU Yang, LIU Shuying. Structure and Pathway Research on Chemical Transformation of Ginsenoside Rb₁ via HPLC-HRMS/MSⁿ/QqQ Technique^† [J]. Chem. J. Chinese Universities, 2017, 38(10): 1730.
[15]	PI Zifeng, WANG Qianqian, ZHANG Jing, Song Fengrui, LIU Zhiqiang. Effect of Schisandra Fruit on Neurochemicals in Hippocampus of Diabetic Encephalopathy Rat Using Online MD-HPLC-MS/MS^† [J]. Chem. J. Chinese Universities, 2015, 36(3): 442.

Chemiluminescence Determination of Brucine,Strychnine and Ephedrines Based on HPLC Separation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments