Interaction of Bovine Serum Albumin with Methyl Orange in Acidic Solution

Abstract

Abstract: The interaction of methyl orange(MO) with bovine serum albumin(BSA)in acidic solution was investigated by a spectrophotometric method. It was considered that the combination of BSAwith MO is due to the noncovalent binding forces.The color change during the binding process is because of the transformation of MO from free-acidic form into bound-basic form.The relationship between absorbance and concentrations of MO species was studied.The Scatchard model was discussed in a new point of view.Two conditional constants,apparent binding constant and maximum binding number,were proposed to express the binding ability of MO on BSA,and two linear regression equations were derived to estimate these two parameters.It was found that sodium chloride concentration and acidity have significant effect on the binding reaction.

Key words: Bovine serum albumin, Methyl orange, Solution equilibria, Theoretical model, Spectrophotometry

TrendMD:

WEI Yong-Ju, LI Ke-An, TONG Shen-Yang. Interaction of Bovine Serum Albumin with Methyl Orange in Acidic Solution[J]. Chem. J. Chinese Universities, 1996, 17(11): 1687.

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Interaction of Bovine Serum Albumin with Methyl Orange in Acidic Solution

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