Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (3): 384.

• Articles • Previous Articles     Next Articles

Studies on the Analysis of β-blockers and Their Metabolites in Urine by Gas Chromatography (Ⅲ)──Analysis of Five β-Blockers and Their Metabolites in Rat'Urine by Solid-phase Extraction and GC/MS

XU You-Xuan, SHEN Li, WANG Shan, WU Yun, ZHANG Chang-Jiu   

  1. China Doping Control Centre, National Research Institute of Sports Medicine, Beijing 100029
  • Received:1994-03-01 Revised:1995-01-05 Online:1995-03-24 Published:1995-03-24

Abstract: Aprocedure for detecting β-blockers was establ ished.Five β-blockers were administrated to rats. The rats' metabolic urine tqamples were hydrolysed with β-glucuronidase,extracted by Sep-Pak C18 columns,and analyzed by GC/MS.Their metabolites were identified by mass spectra and metabolic theories.The recoveries of the drugs were all over 75%.The hydrolysed metliods were compared and the mass fragmentation mechanism was discussed.

Key words: β-blocker, Metabolite, GC/MS

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