Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (2): 206.

• Articles • Previous Articles     Next Articles

FT-Raman Spectroscopy of Triplex DNA d(CT)8·d(AG)8·d(C+T)8

FANG Ye1, WANG Ting1, BAI Chun-Li1, WEI Ying1, TANG You-Qi1, KAN Lou-Sing2   

  1. 1. Institute of Chemistry, Academia Sincia, Beijing, 100080;
    2. Institute of Chemistry, Academia Sinica, Taibei
  • Received:1994-01-31 Revised:1994-10-05 Online:1995-02-24 Published:1995-02-24

Abstract: The conformation of triplex DNAd(CT)8·d(AG)8·d(C+T)8in solution has been investigated by near infrared FT-Raman spectroscopy at 1064 nm. Results clearly revealed that almost all of the sugar pukers of the triplex fall in the normal range for the Bgenus.However,a strong band at 1266 cm-1suggested that the C3'-endo/anti protonated cytosines in the third strand can not be eliminated.

Key words: Triplex DNA, FT-Raman spectra, Conformation

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