CL-ELISA Determination of DNA Antibody in Serum

Abstract

Abstract: Amethod for determination of the DNAantibody in serum was established based on chemiluminescence enzyme-linked immunosorbant assay(CL-ELISA).The DNAantigen is immobilized on the inner surface of polystyrene cup, then reacts with DNAantibody in serum sample, only specific antibody binds to antigen to form Ag-Ab HRPlabeled anti-HuIgGreacts with DNAantibody molecules in the complex to form second Ag-Ab, finally determine the label with p-IPenhanced luminol-H₂O₂-HRPchemiluminescence system.The clinical applications show that present method has a good correlation with ELISA.The correlation coefficient is 0.958.It is more sensitive, convenient and economical than ELISA.

Key words: Chemiluminescence, ELISA, DNA antibody, Systemic lupus erythematosus

TrendMD:

ZHANG Zhu-Jun, ZOU Ke-Wei, FENG Man-Liang. CL-ELISA Determination of DNA Antibody in Serum[J]. Chem. J. Chinese Universities, 1994, 15(6): 832.

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CL-ELISA Determination of DNA Antibody in Serum

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