Enzyme-Amplified Lanthanide Luminescence Spectrometry─Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (12): 1770.

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Enzyme-Amplified Lanthanide Luminescence Spectrometry─Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody

XIE Jian-Wei, YAN Yuan, PENG Xue-Jun, XU Jin-Gou, CHEN Guo-Zhen

Department of Chemistry and Institute of Analytical Chemistry, Xiamen, 361005

Received:1994-05-09 Revised:1994-06-20 Online:1994-12-24 Published:1994-12-24

Abstract

Abstract: Enzyme-amplified lanthanide luminescence (EALL)is a new method which has been recently developed for enzymatically amplified signal detection in ultrasensitive bioanalytical assays where an enzyme is used as label or analysed by itself. In the paper, Horseradish peroxidase (HRP)-Terbium-p-hydroxybenzoic acid system has been studied.The EALLmethod has also been demonstrated for the detection of HRPand tuberculosis antibody.

Key words: Enzyme-amplified lanthanide luminescence spectrometry, Horseradish peroxidase, Tuberculosis antibody

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XIE Jian-Wei, YAN Yuan, PENG Xue-Jun, XU Jin-Gou, CHEN Guo-Zhen. Enzyme-Amplified Lanthanide Luminescence Spectrometry─Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody[J]. Chem. J. Chinese Universities, 1994, 15(12): 1770.

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Enzyme-Amplified Lanthanide Luminescence Spectrometry─Studies on Horseradish Peroxidase-Terbium-p-Hydroxybenzoic Acid System and Its Application to the Determination of Horseradish Peroxidase and Tuberculosis Antibody

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